Overview

  • Product name
  • Description
    Rabbit polyclonal to WBP11
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-290 of Human WBP11 (Uniprot ID: Q9Y2W2).

  • Positive control
    • Jurkat, Raji, K562, THP1 and HL60 whole cell lyastes; Human SkHep1 and Mouse C2C12 xenograft tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab154590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Activates pre-mRNA splicing. May inhibit PP1 phosphatase activity.
  • Tissue specificity
    Ubiquitous. Highly expressed in the heart, pancreas, kidney skeletal muscle, placenta and brain (at protein level). Weakly expressed in liver and lung.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly located in the nucleus with granular heterogeneous distribution. Excluded from nucleoli in interphase cells, distributed throughout cytoplasm in dividing cells. Colocalized with SC35 and U2B in the nucleus. In the cytoplasm, associates with the intermediate filament protein vimentin.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp779M1063 antibody
    • MGC94547 antibody
    • Npw38 binding protein antibody
    • Npw38 binding protein NpwBP antibody
    • Npw38-binding protein antibody
    • NpwBP antibody
    • PPP1R165 antibody
    • Protein phosphatase 1 regulatory subunit 165 antibody
    • SH3 domain binding protein SNP70 antibody
    • SH3 domain-binding protein SNP70 antibody
    • SIPP1 antibody
    • SNP70 antibody
    • Splicing factor PQBP1 and PP1 interacting antibody
    • Splicing factor that interacts with PQBP 1 and PP1 antibody
    • Splicing factor that interacts with PQBP-1 and PP1 antibody
    • WBP 11 antibody
    • WBP-11 antibody
    • Wbp11 antibody
    • WBP11_HUMAN antibody
    • WW domain binding protein 11 antibody
    • WW domain-binding protein 11 antibody
    see all

Images

  • All lanes : Anti-WBP11 antibody (ab154590) at 1/2000 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Raji whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : THP1 whole cell lysate
    Lane 5 : HL60 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 70 kDa
  • Immunohistochemical analysis of paraffin-embedded Human SkHep1 xenograft tissue, labeling WBP11 with ab154590 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Mouse C2C12 xenograft tissue, labeling WBP11 with ab154590 at 1/500 dilution.

References

ab154590 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (endothelial cells)
Total protein in input
1 µg
Immuno-precipitation step
Protein A/G
Specification
endothelial cells
Username

Dr. Orit Uziel

Verified customer

Submitted Dec 12 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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