Anti-WBP11 antibody (ab154590)
Key features and details
- Rabbit polyclonal to WBP11
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-WBP11 antibody
See all WBP11 primary antibodies -
Description
Rabbit polyclonal to WBP11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-290 of Human WBP11 (Uniprot ID: Q9Y2W2).
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Positive control
- Jurkat, Raji, K562, THP1 and HL60 whole cell lyastes; Human SkHep1 and Mouse C2C12 xenograft tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Activates pre-mRNA splicing. May inhibit PP1 phosphatase activity. -
Tissue specificity
Ubiquitous. Highly expressed in the heart, pancreas, kidney skeletal muscle, placenta and brain (at protein level). Weakly expressed in liver and lung. -
Cellular localization
Nucleus. Cytoplasm. Predominantly located in the nucleus with granular heterogeneous distribution. Excluded from nucleoli in interphase cells, distributed throughout cytoplasm in dividing cells. Colocalized with SC35 and U2B in the nucleus. In the cytoplasm, associates with the intermediate filament protein vimentin. - Information by UniProt
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Database links
- Entrez Gene: 51729 Human
- Entrez Gene: 60321 Mouse
- Entrez Gene: 103689936 Rat
- Entrez Gene: 297695 Rat
- Entrez Gene: 414457 Xenopus laevis
- Entrez Gene: 448171 Xenopus tropicalis
- SwissProt: Q9Y2W2 Human
- SwissProt: Q923D5 Mouse
see all -
Alternative names
- DKFZp779M1063 antibody
- MGC94547 antibody
- Npw38 binding protein antibody
see all
Images
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All lanes : Anti-WBP11 antibody (ab154590) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : THP1 whole cell lysate
Lane 5 : HL60 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human SkHep1 xenograft tissue, labeling WBP11 with ab154590 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse C2C12 xenograft tissue, labeling WBP11 with ab154590 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154590 has not yet been referenced specifically in any publications.