Overview

  • Product name
  • Description
    Rabbit polyclonal to WBP11
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (GRRSTSSTKS GKFMNPTDQA RKEARKRELK KNKKQRMMVR AAVLKMKDPK) of Human WBP11 (NP_057396).

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Activates pre-mRNA splicing. May inhibit PP1 phosphatase activity.
  • Tissue specificity
    Ubiquitous. Highly expressed in the heart, pancreas, kidney skeletal muscle, placenta and brain (at protein level). Weakly expressed in liver and lung.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly located in the nucleus with granular heterogeneous distribution. Excluded from nucleoli in interphase cells, distributed throughout cytoplasm in dividing cells. Colocalized with SC35 and U2B in the nucleus. In the cytoplasm, associates with the intermediate filament protein vimentin.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp779M1063 antibody
    • MGC94547 antibody
    • Npw38 binding protein antibody
    • Npw38 binding protein NpwBP antibody
    • Npw38-binding protein antibody
    • NpwBP antibody
    • PPP1R165 antibody
    • Protein phosphatase 1 regulatory subunit 165 antibody
    • SH3 domain binding protein SNP70 antibody
    • SH3 domain-binding protein SNP70 antibody
    • SIPP1 antibody
    • SNP70 antibody
    • Splicing factor PQBP1 and PP1 interacting antibody
    • Splicing factor that interacts with PQBP 1 and PP1 antibody
    • Splicing factor that interacts with PQBP-1 and PP1 antibody
    • WBP 11 antibody
    • WBP-11 antibody
    • Wbp11 antibody
    • WBP11_HUMAN antibody
    • WW domain binding protein 11 antibody
    • WW domain-binding protein 11 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling WBP11 with ab85563 at 1/100. A Cy3-conjugate donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of the cell bodies of pinealocytes and their processes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - WBP11. Right - Merge.
  • Anti-WBP11 antibody (ab85563) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • All lanes : Anti-WBP11 antibody (ab85563) at 2 µg/ml

    Lane 1 : Human NT2 cells
    Lane 2 : Mouse WT brain lysate


    Predicted band size : 70 kDa
    WB Suggested Anti-Tdp1 antibody (ab113896) Titration: 2 µg/ml Positive Control: H292 and H226 cells

References

ab85563 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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