The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250. The staining pattern is dependent on the antibody titer and nature of the biology. Proliferating tumor cells might be positive for nuclear staining while the differentiated cells show no nuclear staining. Antibody titer and antigen retrieval time should be adjusted.
Use at an assay dependent concentration. PubMed: 24086612
Tissue specificityStrongly expressed in heart, skeletal muscle and kidney. Also expressed in spleen, liver, lung and testis.
Involvement in diseaseNote=WBSCR22 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR22 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Sequence similaritiesBelongs to the methyltransferase superfamily.