The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250. The staining pattern is dependent on the antibody titer and nature of the biology. Proliferating tumor cells might be positive for nuclear staining while the differentiated cells show no nuclear staining. Antibody titer and antigen retrieval time should be adjusted.
Use at an assay dependent concentration. PubMed: 24086612
Strongly expressed in heart, skeletal muscle and kidney. Also expressed in spleen, liver, lung and testis.
Involvement in disease
Note=WBSCR22 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR22 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Zorbas C et al. The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis. Mol Biol Cell26:2080-95 (2015).
Read more (PubMed: 25851604) »
Ounap K et al. The Human WBSCR22 Protein Is Involved in the Biogenesis of the 40S Ribosomal Subunits in Mammalian Cells. PLoS One8:e75686 (2013).
Read more (PubMed: 24086612) »