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Tissue/ cell preparation: Con A stimulated bovine lymphocytes.
This clone (CC101) immunoprecipitates a 215 kD molecule from bovine cells, and also recognises the swine homologue of WC1, which is a 180kD molecule. In pigs the 180kD molecule is expressed by a gamma/delta TCR positive T cell population that is also CD2, CD4, CD8. Weak results in flow cytometry.
Our Abpromise guarantee covers the use of ab34050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
Use 10µl of the suggested working dilution for 106 cells in 100µl.
ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/10 - 1/50.|
|IP||Use at an assay dependent concentration.|
ab34050 has not yet been referenced specifically in any publications.