Anti-WDFY3 antibody (ab84888)


  • Product nameAnti-WDFY3 antibody
  • Description
    Rabbit polyclonal to WDFY3
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 500 and 550 of human WDFY3 (accession number: NP_055806.2)

  • Positive control
    • Human Controls: Breast Carcinoma, Ovarian Carcinoma, Placenta, Prostate Carcinoma


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified using an epitope specific to WDFY3 immobilized on solid support
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab84888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.
ICC/IF Use at an assay dependent concentration.


  • RelevanceWDFY3 ( WD repeat and FYVE domain containing protein 3) was identified in a screen for phosphatidylinositol 3 phosphate [PtdIns(3)P] binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3) binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein (Alfy).
  • Cellular localizationMembrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells.
  • Database links
  • Alternative names
    • Alfy antibody
    • Autophagy linked FYVE protein antibody
    • KIAA0993 antibody
    • MGC16461 antibody
    • WD repeat and FYVE domain containing 3 antibody
    • ZFYVE25 antibody
    see all

Anti-WDFY3 antibody images

  • Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human ovarian carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
  • Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human breast carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
  • ab84888 staining WDFY3 in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.5% in PBS and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 1 hour at 25°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit monoclonal (1/2000) was used as the secondary antibody.

    See Abreview

References for Anti-WDFY3 antibody (ab84888)

ab84888 has not yet been referenced specifically in any publications.

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To our knowledge, ab84888 has not been tested in WB. However by participating in our AbTrial program you can now use our products in an untested application or species without finan...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa (Cervical Epithelium Carcinoma))
Specification HeLa (Cervical Epithelium Carcinoma)
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton X-100 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

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Submitted Sep 08 2010