Anti-WDFY3 antibody (ab84888)

Overview

  • Product name
    Anti-WDFY3 antibody
  • Description
    Rabbit polyclonal to WDFY3
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 500 and 550 of human WDFY3 (accession number: NP_055806.2)

  • Positive control
    • Human Controls: Breast Carcinoma, Ovarian Carcinoma, Placenta, Prostate Carcinoma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified using an epitope specific to WDFY3 immobilized on solid support
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    WDFY3 ( WD repeat and FYVE domain containing protein 3) was identified in a screen for phosphatidylinositol 3 phosphate [PtdIns(3)P] binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3) binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein (Alfy).
  • Cellular localization
    Membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells.
  • Database links
  • Alternative names
    • Alfy antibody
    • Autophagy linked FYVE protein antibody
    • KIAA0993 antibody
    • MGC16461 antibody
    • WD repeat and FYVE domain containing 3 antibody
    • ZFYVE25 antibody
    see all

Anti-WDFY3 antibody images

  • Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human ovarian carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
  • Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human breast carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
  • ab84888 staining WDFY3 in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.5% in PBS and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 1 hour at 25°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit monoclonal (1/2000) was used as the secondary antibody.

    See Abreview

References for Anti-WDFY3 antibody (ab84888)

ab84888 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your interest in our products.


To our knowledge, ab84888 has not been tested in WB. However by participating in our AbTrial program you can now use our products in an untested application or species without finan...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa (Cervical Epithelium Carcinoma))
Specification
HeLa (Cervical Epithelium Carcinoma)
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up