Overview

  • Product name
  • Description
    Rabbit polyclonal to WDHD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Orangutan
  • Immunogen

    A synthetic peptide of a region between residues 975 and 1025 of human WDHD1.

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab72429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notes
    IP: Use at a concentration of 2 - 5 µg/mg lysate.
    Is unsuitable for WB.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The protein WDHD1 contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms.
    • Cellular localization
      Nuclear; nucleoplasm.
    • Database links
    • Alternative names
      • Acidic nucleoplasmic DNA binding protein 1 antibody
      • And 1 antibody
      • And1 antibody
      • WD repeat and HMG box DNA binding protein 1 antibody
      • WDHD 1 antibody
      see all

    Images

    • Immunoprecipitation of WDHD1 from 1mg whole HeLa cells lysate by 3 ug ab72429 followed by WB (20% of IP loaded):
      Lane 1: ab72429 precipitate

      Lane 2: ab72436 precipitate

      Lane 3: Control IgG

      For blotting immunoprecipitated WDHD1, ab72436 was used at 1 ug/ml.

      Detection: ReliaBLOT® Reagents and Procedures were incorporated followed by chemiluminescence with an exposure time of 10 seconds.

    References

    ab72429 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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