• Product nameAnti-WDHD1 antibody
    See all WDHD1 primary antibodies
  • Description
    Rabbit polyclonal to WDHD1
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    A synthetic peptide corresponding to a region between residues 1079 and 1129 of human WDHD1.

  • Positive control
    • HeLa whole cell lysate



Our Abpromise guarantee covers the use of ab72436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500. Use with methanol fixed cells.
WB 1/2000 - 1/10000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
IP Use a concentration of 2 - 5 µg/ml.


  • RelevanceThe protein WDHD1 contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms.
  • Cellular localizationNuclear; nucleoplasm.
  • Database links
  • Alternative names
    • Acidic nucleoplasmic DNA binding protein 1 antibody
    • And 1 antibody
    • And1 antibody
    • WD repeat and HMG box DNA binding protein 1 antibody
    • WDHD 1 antibody
    see all

Anti-WDHD1 antibody images

  • Immunoprecipitation of WDHD1 from 1mg whole HeLa cells lysate by 3 ug ab72436 followed by WB (20% of IP loaded):
    Lane 1: ab72429 precipitate

    Lane 2: ab72436 precipitate

    Lane 3: Control IgG

    For blotting immunoprecipitated WDHD1, ab72436 was used at 1 ug/ml.

    Detection: Chemiluminescence with an exposure time of 10 seconds.
  • All lanes : Anti-WDHD1 antibody (ab72436) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size : 126 kDa
    Observed band size : 126 kDa
  • ab72436 (1/500) staining WDHD1 in assynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

    See Abreview

References for Anti-WDHD1 antibody (ab72436)

This product has been referenced in:
  • Zhou Y  et al. Role of WDHD1 in Human Papillomavirus-Mediated Oncogenesis Identified by Transcriptional Profiling of E7-Expressing Cells. J Virol 90:6071-84 (2016). Read more (PubMed: 27099318) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No

Dr. Kirk Mcmanus

Verified customer

Submitted Mar 15 2011