Overview

  • Product nameAnti-WDR20 antibody
    See all WDR20 primary antibodies
  • Description
    Mouse monoclonal to WDR20
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human, Kangaroo, Non Human Primates, Snake, Macaque Monkey, Deer, Plasmodium falciparum, Staphylococcus aureus, Syrian Hamster, Dragon Fly, Electric Eel (Electrophorus electricus), Hoolock Gibbon, Lethocerus indicus (Waterbug)
  • Immunogen

    Recombinant full length (aa 1- 570) human WDR20 protein with tag (AAH28387)

  • Positive control
    • WDR20 transfected 293T cell lysate; recombinant WDR20.

Properties

Applications

Our Abpromise guarantee covers the use of ab68356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similaritiesContains 5 WD repeats.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686B2131 antibody
      • DMR antibody
      • FLJ27314 antibody
      • FLJ33659 antibody
      • MGC33177 antibody
      • MGC33183 antibody
      • Protein DMR antibody
      • WD repeat domain 20 antibody
      • WD repeat ontaining protein 20 antibody
      • WD repeat protein 20 antibody
      • WD repeat-containing protein 20 antibody
      • WDR 20 antibody
      • Wdr20 antibody
      • WDR20_HUMAN antibody
      see all

    Anti-WDR20 antibody images

    • Anti-WDR20 antibody (ab68356) at 1/500 dilution + recombinant WDR20 at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 63 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-WDR20 antibody (ab68356) at 1/500 dilution

      Lane 1 : WDR20 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 63 kDa
      Observed band size : 63 kDa

    References for Anti-WDR20 antibody (ab68356)

    ab68356 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68356.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"