Anti-WDR26 antibody (ab85962)
Key features and details
- Rabbit polyclonal to WDR26
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WDR26 antibody
See all WDR26 primary antibodies -
Description
Rabbit polyclonal to WDR26 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide within a region between residue 600 and 650 of human WDR26 (BAG10986.1).
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Positive control
- Hela whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85962 was affinity purified using an epitope specific to WDR26 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.4 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 72 kDa).
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Notes |
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WB
Use a concentration of 0.4 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 72 kDa). |
Target
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Relevance
The WDR26 gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids, which are typically bracketed by gly-his and trp-asp (GH-WD). WD repeats may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Two transcript variants encoding two different isoforms have been found for this gene. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 80232 Human
- Entrez Gene: 226757 Mouse
- Entrez Gene: 498301 Rat
- Entrez Gene: 733878 Xenopus tropicalis
- Entrez Gene: 564794 Zebrafish
- SwissProt: Q9H7D7 Human
- SwissProt: Q8C6G8 Mouse
- SwissProt: Q28D01 Xenopus tropicalis
see all -
Alternative names
- CDW2 antibody
- CUL4 and DDB1 associated WDR protein 2 antibody
- GID complex subunit 7 homolog antibody
see all
Images
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All lanes : Anti-WDR26 antibody (ab85962) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 220 kDa, 50 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab85962 has been referenced in 2 publications.
- Soliman SHA et al. Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. Sci Rep 10:7138 (2020). PubMed: 32346083
- Maitland MER et al. The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation. Sci Rep 9:9864 (2019). PubMed: 31285494