Anti-WDR3 antibody (ab176817)
Key features and details
- Rabbit polyclonal to WDR3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WDR3 antibody -
Description
Rabbit polyclonal to WDR3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human WDR3 aa 225-275. The exact sequence is proprietary. NP_006775.1.
Sequence:EIEDPEEPDPKKIKGSSPGIQDTLEAEDGAFETDEAPEDRILSCRKAGSI M
Database link: Q9UNX4 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab176817 was affinity purified using an epitope specific to WDR3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 106 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 106 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
This gene encodes a nuclear protein containing 10 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, which usually include a trp-asp at the C terminal end. Proteins belonging to the WD repeat family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. -
Cellular localization
Nucleus, nucleolus. -
Database links
- Entrez Gene: 469436 Chimpanzee
- Entrez Gene: 101135112 Gorilla
- Entrez Gene: 10885 Human
- Entrez Gene: 100458846 Orangutan
- Omim: 604737 Human
- SwissProt: Q9UNX4 Human
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Alternative names
- dJ776P7.2 (WD repeat domain 3) antibody
- FLJ12796 antibody
- WD repeat containing protein 3 antibody
see all
Images
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All lanes : Anti-WDR3 antibody (ab176817) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 106 kDa
Exposure time: 30 seconds -
Detection of WDR3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176817 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176817 was used at 0.1 µg/ml (Lane 2) represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab176817 has been referenced in 1 publication.
- Su W et al. Overexpressed WDR3 induces the activation of Hippo pathway by interacting with GATA4 in pancreatic cancer. J Exp Clin Cancer Res 40:88 (2021). PubMed: 33648545