Anti-WDR33 antibody (ab72115)
Key features and details
- Rabbit polyclonal to WDR33
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WDR33 antibody
See all WDR33 primary antibodies -
Description
Rabbit polyclonal to WDR33 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to a region between residues 1286 and 1336 of human WDR33 (NP_060853.3).
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72115 was affinity purified using an epitope specific to WDR33 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 146 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 146 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Tissue specificity
Most highly expressed in testis. -
Sequence similarities
Contains 1 collagen-like domain.
Contains 7 WD repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55339 Human
- SwissProt: Q6NUQ0 Human
- SwissProt: Q9C0J8 Human
- Unigene: 554831 Human
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Alternative names
- WD repeat containing protein 33 antibody
- WD repeat domain 33 antibody
- WD repeat-containing protein 33 antibody
see all
Images
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All lanes : Anti-WDR33 antibody (ab72115) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 146 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Detection of WDR33 by Western Blot of Immunprecipitate.
ab72115 at 1µg/ml staining WDR33 in HeLa whole cell lysate immunoprecipitated using ab72115 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab72115 has not yet been referenced specifically in any publications.