Overview

  • Product name
  • Description
    Rabbit polyclonal to WDR33
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1286 and 1336 of human WDR33 (NP_060853.3).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab72115 was affinity purified using an epitope specific to WDR33 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 146 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Tissue specificity
    Most highly expressed in testis.
  • Sequence similarities
    Contains 1 collagen-like domain.
    Contains 7 WD repeats.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • WD repeat containing protein 33 antibody
    • WD repeat domain 33 antibody
    • WD repeat-containing protein 33 antibody
    • WD repeat-containing protein WDC146 antibody
    • WDC146 antibody
    • WDR 33 antibody
    • WDR33 antibody
    • WDR33_HUMAN antibody
    see all

Images

  • All lanes : Anti-WDR33 antibody (ab72115) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml
    Lane 4 : 293T whole cell lysate at 50 µg/ml

    Developed using the ECL technique

    Predicted band size : 146 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
  • Detection of WDR33 by Western Blot of Immunprecipitate.
    ab72115 at 1µg/ml staining WDR33 in HeLa whole cell lysate immunoprecipitated using ab72115 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab72115 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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