Overview

  • Product name
    Anti-WDR34 antibody
  • Description
    Rabbit polyclonal to WDR34
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 431-480 (QLSLKYLFAV RWSPVRPLVF AAASGKGDVQ LFDLQKSSQK PTVLIKQTQD) of human WDR34 (NP_443076).

  • Positive control
    • Fetal Brain lysate. IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab81030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • Function
    Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Inhibits the MAP3K7-induced NF-kappa-B activation pathway. Inhibits MAP3K7 phosphorylation at 'Thr-184' and 'Thr-187' upon Il-1 beta stimulation.
  • Tissue specificity
    Expressed in several cell lines (at protein level).
  • Sequence similarities
    Contains 5 WD repeats.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA216B9.3 antibody
    • DIC5 antibody
    • MGC20486 antibody
    • OTTHUMP00000022282 antibody
    • RP11 216B9.5 antibody
    • WD repeat containing protein 34 antibody
    • WD repeat domain 34 antibody
    • WD repeat-containing protein 34 antibody
    • WDR 34 antibody
    • Wdr34 antibody
    • WDR34_HUMAN antibody
    see all

Images

  • ICC/IF image of ab81030 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81030, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-WDR34 antibody (ab81030) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 12%

References

This product has been referenced in:
  • Taylor SP  et al. Mutations in DYNC2LI1 disrupt cilia function and cause short rib polydactyly syndrome. Nat Commun 6:7092 (2015). WB, IF . Read more (PubMed: 26077881) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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