Overview

  • Product nameAnti-WDR4 antibody
    See all WDR4 primary antibodies
  • Description
    Rabbit polyclonal to WDR4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 2 and 189 of Human WDR4 (AAH01074; UniProt ID: P57081 isoform 3).

  • Positive control
    • A549 whole cell lysate; NCIN87 xenograft tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab102471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 for isoforms 1 , 2; for isoform 3, 30 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0).

Target

  • FunctionRequired for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational change of the catalytic subunit.
  • PathwaytRNA modification; N(7)-methylguanine-tRNA biosynthesis.
  • Sequence similaritiesBelongs to the WD repeat TRM82 family.
    Contains 4 WD repeats.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000109401 antibody
    • OTTHUMP00000109402 antibody
    • TRM82 antibody
    • tRNA (guanine N(7) ) methyltransferase subunit WDR4 antibody
    • tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 antibody
    • WD repeat containing protein 4 antibody
    • WD repeat protein 4 antibody
    • WD repeat protein domain 4 protein isoform 2 antibody
    • WD repeat-containing protein 4 antibody
    • wdr4 antibody
    • WDR4_HUMAN antibody
    see all

Anti-WDR4 antibody images

  • Anti-WDR4 antibody (ab102471) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 45 for isoforms 1 , 2; for isoform 3, 30 kDa
  • ab102471, at a 1/500 dilution, staining WDR4 in paraffin embedded NCIN87 xenograft by Immunohistochemistry.

References for Anti-WDR4 antibody (ab102471)

ab102471 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"