Overview

  • Product nameAnti-WDR4 antibody
    See all WDR4 primary antibodies
  • Description
    Rabbit polyclonal to WDR4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region within the N-terminal amino acids 35-84 (FIYDCSAAEK KSQENKGEDA PLDQGSGAIL ASTFSKSGSY FALTDDSKRL) of Human WDR4 (NP_061139).

  • Positive control
    • Human Placenta lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.

Target

  • FunctionRequired for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational change of the catalytic subunit.
  • PathwaytRNA modification; N(7)-methylguanine-tRNA biosynthesis.
  • Sequence similaritiesBelongs to the WD repeat TRM82 family.
    Contains 4 WD repeats.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000109401 antibody
    • OTTHUMP00000109402 antibody
    • TRM82 antibody
    • tRNA (guanine N(7) ) methyltransferase subunit WDR4 antibody
    • tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 antibody
    • WD repeat containing protein 4 antibody
    • WD repeat protein 4 antibody
    • WD repeat protein domain 4 protein isoform 2 antibody
    • WD repeat-containing protein 4 antibody
    • wdr4 antibody
    • WDR4_HUMAN antibody
    see all

Anti-WDR4 antibody images

References for Anti-WDR4 antibody (ab83552)

ab83552 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"