Anti-WDR43 antibody - C-terminal (ab174906)

Overview

  • Product name
    Anti-WDR43 antibody - C-terminal
    See all WDR43 primary antibodies
  • Description
    Rabbit polyclonal to WDR43 - C-terminal
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human WDR43 aa 643-673 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q15061

  • Positive control
    • Jurkat and HepG2 cell lysate; Human cervical carcinoma tissue; HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab174906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 75 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Sequence similarities
    Belongs to the UTP5 family.
    Contains 6 WD repeats.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0007 antibody
    • NET12 antibody
    • UTP5 antibody
    • UTP5, small subunit (SSU) processome component, homolog antibody
    • WD repeat domain 43 antibody
    • WD repeat-containing protein 43 antibody
    • WDR43 antibody
    • WDR43_HUMAN antibody
    see all

Images

  • All lanes : Anti-WDR43 antibody - C-terminal (ab174906) at 1/100 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 75 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human carevical carcinoma tissue labeling WDR43 with ab174906 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of HepG2 cells labeling EDR43 with ab174906 at 1/10 dilution (bottom histogram), compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab174906 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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