Overview

  • Product nameAnti-WDR48 antibody
  • Description
    Rabbit polyclonal to WDR48
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    antigen sequence: SIIQCHILND KRHILTKDTN NNVAYWDVLK ACKVEDLGKV DFEDEIKKRF KMVYVPNWFS VDLKTGMLTI TLDESDCFAA WVSAKDAGF, corresponding to internal sequence amino acids 362-450 of Human WDR48.

  • Positive control
    • Human colon tissue; RT 4, U51 MG, Human liver and Human tonsil lysates; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab122473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • FunctionRegulator of deubiquitinating complexes. Acts as a strong activator of USP1 by enhancing the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Also activates deubiquitinating activity of complexes containing USP12 and USP46, respectively. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the WD repeat WDR48 family.
    Contains 8 WD repeats.
  • DomainN-terminal WD region interacts with TIP and C-terminal region mediates lysosomal localization. The WD repeats are required for the interaction with deubiquitinating enzymes USP1, USP12 and USP46.
  • Cellular localizationNucleus. Cytoplasm. Lysosome. Mainly cytoplasmic. In case of infection by papillomavirus HPV11, translocates to the nucleus via its interaction with papillomavirus HPV11.
  • Information by UniProt
  • Database links
  • Alternative names
    • p80 antibody
    • UAF 1 antibody
    • UAF1 antibody
    • USP1 associated factor 1 antibody
    • USP1-associated factor 1 antibody
    • WD repeat containing protein 48 antibody
    • WD repeat domain 48 antibody
    • WD repeat endosomal protein antibody
    • WD repeat-containing protein 48 antibody
    • WDR 48 antibody
    • WDR48 antibody
    • WDR48_HUMAN antibody
    see all

Anti-WDR48 antibody images

  • All lanes : Anti-WDR48 antibody (ab122473) at 1/100 dilution

    Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
    Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • ab122473, at 1/20 dilution, staining WDR48 in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • All lanes : Anti-WDR48 antibody (ab122473) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique
  • ab122473, at 4 µg/ml, staining WDR48 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

References for Anti-WDR48 antibody (ab122473)

ab122473 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"