Overview

  • Product nameAnti-WDR5 antibody
    See all WDR5 primary antibodies
  • Description
    Rabbit polyclonal to WDR5
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Chicken, Cow, Xenopus laevisDoes not react with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human WDR5.

    (Peptide available as ab24388.)

  • Positive control
    • This antibody gave a positive signal in western blot in the follwoing whole cell lysates: HeLa; A549; NIH3T3. This antibody gave a positive signal in the following Formaldehyde fixed cell lines: HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab22512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionContributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
  • Sequence similaritiesBelongs to the WD repeat WDR5/wds family.
    Contains 7 WD repeats.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410008O07Rik antibody
    • AA408785 antibody
    • AA960360 antibody
    • BIG 3 antibody
    • Big antibody
    • BIG3 antibody
    • BMP2 induced 3 kb gene protein antibody
    • BMP2 induced gene, 3-KB antibody
    • BMP2-induced 3-kb gene protein antibody
    • MGC114572 antibody
    • OTTHUMP00000162494 antibody
    • SWD 3 antibody
    • SWD3 antibody
    • SWD3 Set1c WD40 repeat protein homolog antibody
    • WD repeat containing protein 5 antibody
    • WD repeat domain 5 antibody
    • WD repeat protein 5 antibody
    • WD repeat-containing protein 5 antibody
    • WD repeat-containing protein BIG-3 antibody
    • WDR 5 antibody
    • Wdr5 antibody
    • WDR5_HUMAN antibody
    see all

Anti-WDR5 antibody images

  • All lanes : Anti-WDR5 antibody (ab22512) at 1 µg/ml (blocked with 5% Milk)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab22512 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab22512 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-WDR5 antibody (ab22512) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

References for Anti-WDR5 antibody (ab22512)

This product has been referenced in:
  • Shinsky SA  et al. Biochemical Reconstitution and Phylogenetic Comparison of Human SET1 Family Core Complexes Involved in Histone Methylation. J Biol Chem N/A:N/A (2015). Read more (PubMed: 25561738) »
  • Vulto-van Silfhout AT  et al. Variants in CUL4B are associated with cerebral malformations. Hum Mutat 36:106-17 (2015). Read more (PubMed: 25385192) »

See all 16 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab22512.

To check the status of the order please contact o...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 40 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (10-20%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Apr 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Kidney)
Loading amount 40 µg
Specification Kidney
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Dec 08 2011

Thank you for your enquiry. I am sorry to hear ab22512 is giving you trouble. I don't have a specific protocol to use with this antibody but if you could let me know some more about your sample preparation and experimental protocol I'd be happy to offe...

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Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - nuclear (nuclear)
Loading amount 10 µg
Specification nuclear
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"