Overview

  • Product name
  • Description
    Rabbit polyclonal to WDR58
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 144-193 (AGGDCQLHTM DLETGTFTRV LRGHTDYIHC LALRERSPEV LSGGEDGAVR) of human WDR58 (NP_077315).

  • Positive control
    • Human fetal pancreas lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
  • Sequence similarities
    Belongs to the WD repeat THOC6 family.
    Contains 7 WD repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • fSAP35 antibody
    • Functional spliceosome-associated protein 35 antibody
    • MGC2655 antibody
    • PSEC0006 antibody
    • THO complex 6 homolog antibody
    • THO complex subunit 6 homolog antibody
    • Thoc6 antibody
    • THOC6_HUMAN antibody
    • WD repeat containing protein 58 antibody
    • WD repeat domain 58 antibody
    • WD repeat-containing protein 58 antibody
    see all

Images

  • Anti-WDR58 antibody (ab84900) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal pancreas lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

References

ab84900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84900.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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