Overview

  • Product nameAnti-WDR66 antibody
    See all WDR66 primary antibodies
  • Description
    Rabbit polyclonal to WDR66
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide within residues: GELEEKTDRM PQDELGQERR DLEPENREEG QERRVSDIQS KAGISRESLV , corresponding to N terminal amino acids 179-228 of Human WDR66 (NP_653269).

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 168 kDa (predicted molecular weight: 131 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

Anti-WDR66 antibody images

  • Anti-WDR66 antibody (ab83694) at 1 µg/ml (5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 131 kDa
    Observed band size : 168 kDa (why is the actual band size different from the predicted?)

References for Anti-WDR66 antibody (ab83694)

ab83694 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"