Overview

  • Product name
    Anti-WDR67 antibody
  • Description
    Rabbit polyclonal to WDR67
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: PLLSCNLKDD FEFFFHHRNN LDINVVIRQV YHLMETTPTD IHPDSMLNVF VALTKGQYPV FNQYPKFIVD YQTQERERIR NDELDYLRER QTVEDMQAKV, corresponding to amino acids 617-716 of Human WDR67.

  • Positive control
    • Human testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB Use at an assay dependent concentration. Predicted molecular weight: 124 kDa.

Target

Images

  • Lane 1 :
    Lane 2 :
    Lane 3 :

    Lane 1 : Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
    Lane 2 : Negative control (vector only transfected HEK293T lysate)
    Lane 3 : Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407919)


    Predicted band size : 124 kDa
  • Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab121771 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121771 at 1/50 dilution staining WDR67 in paraffin embedded Human testis tissue by Immunohistochemistry.

References

ab121771 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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