The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
FunctionInvolved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (By similarity). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the WD repeat DCAF7 family. Contains 4 WD repeats.
Cellular localizationCytoplasm. Nucleus. Overexpression of DIAHP1 or active RHOA causes translocation from the nucleus to cytoplasm.