Overview

  • Product nameAnti-WDR70 antibody
  • Description
    Rabbit polyclonal to WDR70
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 604 and 654 of Human WDR70 (NP_060504.1)

  • Positive control
    • HeLa or 293T whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab86366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Sequence similaritiesBelongs to the WD repeat GAD-1 family.
    Contains 7 WD repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4833422F06Rik antibody
    • FLJ10233 antibody
    • MGC109151 antibody
    • RGD1309487 antibody
    • WD repeat containing protein 70 antibody
    • WD repeat domain 70 antibody
    • WD repeat-containing protein 70 antibody
    • wdr70 antibody
    • WDR70_HUMAN antibody
    see all

Anti-WDR70 antibody images

  • All lanes : Anti-WDR70 antibody (ab86366) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 73 kDa


    Exposure time : 30 seconds
  • Detection of WDR70 by Western Blot of Immunprecipitate.
    ab86366 at 1µg/ml staining WDR70 in HeLa whole cell lysate immunoprecipitated using ab86366 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-WDR70 antibody (ab86366)

ab86366 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Permeabilization Yes - 0.5%TritonX
Specification U2OS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative Paraformaldehyde
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Submitted Sep 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"