• Product nameAnti-WDR77 antibody
    See all WDR77 primary antibodies
  • Description
    Mouse monoclonal to WDR77
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-343 of Human WDR77


Our Abpromise guarantee covers the use of ab57722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNon-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
    • Tissue specificityHighly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.
    • Sequence similaritiesContains 5 WD repeats.
    • Developmental stageExpressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation.
    • Cellular localizationNucleus. Cytoplasm. Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2610312E17Rik antibody
      • Androgen receptor cofactor p44 antibody
      • C79984 antibody
      • HKMT1069 antibody
      • MEP 50 antibody
      • MEP-50 antibody
      • MEP50 antibody
      • MEP50_HUMAN antibody
      • Methylosome protein 50 antibody
      • MGC2722 antibody
      • Nbla10071 antibody
      • p44 antibody
      • p44/Mep50 antibody
      • RGD1310479 antibody
      • RP11 552M11.3 antibody
      • WD repeat containing protein 77 antibody
      • WD repeat domain 77 antibody
      • WD repeat-containing protein 77 antibody
      • WDR77 antibody
      see all

    Anti-WDR77 antibody images

    • WDR77 antibody (ab57722) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.

    • Predicted band size : 37 kDa
      WDR77 antibody (ab57722) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-WDR77 antibody (ab57722)

    This product has been referenced in:
    • Tsutsui T  et al. Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune response genes. J Biol Chem 288:20955-65 (2013). Read more (PubMed: 23749998) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293)
    Loading amount 60 µg
    Specification HEK293
    Gel Running Conditions Reduced Denaturing (12,5)
    Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: RT°C

    Mr. Jörg Hoehfeld

    Verified customer

    Submitted Mar 14 2008