Overview

  • Product nameAnti-WDR77 antibody
    See all WDR77 primary antibodies
  • Description
    Rabbit polyclonal to WDR77
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide within Human WDR77 aa 1-50. The exact sequence is proprietary.
    Database link: NP_077007.1

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionNon-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
    • Tissue specificityHighly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.
    • Sequence similaritiesContains 5 WD repeats.
    • Developmental stageExpressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation.
    • Cellular localizationNucleus. Cytoplasm. Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2610312E17Rik antibody
      • Androgen receptor cofactor p44 antibody
      • C79984 antibody
      • HKMT1069 antibody
      • MEP 50 antibody
      • MEP-50 antibody
      • MEP50 antibody
      • MEP50_HUMAN antibody
      • Methylosome protein 50 antibody
      • MGC2722 antibody
      • Nbla10071 antibody
      • p44 antibody
      • p44/Mep50 antibody
      • RGD1310479 antibody
      • RP11 552M11.3 antibody
      • WD repeat containing protein 77 antibody
      • WD repeat domain 77 antibody
      • WD repeat-containing protein 77 antibody
      • WDR77 antibody
      see all

    Anti-WDR77 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic islet cell tumor tissue labelling WDR77 with ab80616 at 1/5000 (0.2µg/ml). Detection: DAB.

    References for Anti-WDR77 antibody (ab80616)

    ab80616 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"