1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionEssential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, it controls telomerase localization to Cajal body. Required for delivery of TERC to telomeres during S phase and for telomerase activity. Binds small Cajal body RNAs (scaRNAs). The mRNA encoding this protein plays a critical role in the regulation of p53 expression at the post-transcriptional level; it is involved both in maintaining basal p53 mRNA levels and in p53 induction upon DNA damage.
Tissue specificityExpressed in all tissues and cell lines examined.
Involvement in diseaseDefects in WRAP53 are the cause of dyskeratosis congenita autosomal recessive type 3 (DKCB3) [MIM:613988]. A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Sequence similaritiesContains 6 WD repeats.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling WDR79 with ab99376 at 1/200 (1µg/ml). Detection: DAB.
Western blot - WDR79 antibody (ab99376)
All lanes : Anti-WDR79 antibody (ab99376) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 59 kDa
Exposure time : 30 seconds
Immunoprecipitation - WDR79 antibody (ab99376)
Lane 1 : Detection of Human WDR79 by Immunoprecipitation from HeLa cells(1 mg for IP, 20% of IP loaded), using 3µg/mg lysate .
Lane 2 : Control IgG
References for Anti-WDR79 antibody (ab99376)
This product has been referenced in:
Yuan P et al. Telomerase Cajal body protein 1 depletion inhibits telomerase trafficking to telomeres and induces G1 cell cycle arrest in A549 cells. Oncol Lett8:1009-1016 (2014).
Read more (PubMed: 25120649) »
Jády BE et al. Human intron-encoded Alu RNAs are processed and packaged into Wdr79-associated nucleoplasmic box H/ACA RNPs. Genes Dev26:1897-910 (2012).
Read more (PubMed: 22892240) »