Anti-WDR82 antibody - N-terminal (ab175071)

Overview

  • Product name
    Anti-WDR82 antibody - N-terminal
    See all WDR82 primary antibodies
  • Description
    Rabbit polyclonal to WDR82 - N-terminal
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Zebrafish, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human WDR82 aa 2-31 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6UXN9

  • Positive control
    • HepG2 cell line lysate; Human brain tissue; HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 35 kDa.

Target

  • Function
    Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
  • Sequence similarities
    Belongs to the WD repeat SWD2 family.
    Contains 6 WD repeats.
  • Cellular localization
    Nucleus. Associates with chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • TMEM113 antibody
    • MST107 antibody
    • MSTP107 antibody
    • PRO2730 antibody
    • PRO34047 antibody
    • Protein TMEM113 antibody
    • Swd2 antibody
    • transmembrane protein 113 antibody
    • UNQ9342/PRO34047 antibody
    • WD repeat domain 82 antibody
    • WD repeat-containing protein 82 antibody
    • wdr82 antibody
    • WDR82_HUMAN antibody
    • WDR82A antibody
    see all

Images

  • Anti-WDR82 antibody - N-terminal (ab175071) at 1/100 dilution + HepG2 cell line lysate at 35 µg

    Predicted band size : 35 kDa
  • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling WDR82 with ab175071 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometrical analysis of HepG2 cells with ab175071 antibody at a dilution of 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175071 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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