Overview

  • Product name
  • Description
    Rabbit polyclonal to WDR85
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 212-418 of Human WDR85 (AAH17335).

  • Positive control
    • IMR32 cell lysate. Gastric carcinoma. tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) may alternatively be used for heat-mediated antigen retrieval.

Target

  • Function
    Plays a role in the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.
  • Sequence similarities
    Belongs to the WD repeat WDR85 family.
    Contains 3 WD repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • C9orf112 antibody
    • Diphthamide biosynthesis 7 antibody
    • DPH7 antibody
    • FLJ90634 antibody
    • OTTHUMP00000022705 antibody
    • RP11-48C7.3 antibody
    • RRT2 antibody
    • WD repeat domain 85 antibody
    • WD repeat-containing protein 85 antibody
    • wdr85 antibody
    • WDR85_HUMAN antibody
    see all

Images

  • Anti-WDR85 antibody (ab111910) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size : 51 kDa
  • ab111910 at 1/500 dilution staining WDR85 in paraffin-embedded gastric carcinoma by Immunohistochemistry.

References

ab111910 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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