1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) may alternatively be used for heat-mediated antigen retrieval.
FunctionPlays a role in the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.
Sequence similaritiesBelongs to the WD repeat WDR85 family. Contains 3 WD repeats.