Overview

  • Product name
  • Description
    Rabbit polyclonal to WDR85
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 340-430 of Human WDR85 (UniProt ID: Q9BTV6)

  • Positive control
    • Human breast tissue, U-251 MG cell lysate, RT-4 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab150815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500. Predicted molecular weight: 51 kDa.

Target

  • Function
    Plays a role in the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.
  • Sequence similarities
    Belongs to the WD repeat WDR85 family.
    Contains 3 WD repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • C9orf112 antibody
    • Diphthamide biosynthesis 7 antibody
    • DPH7 antibody
    • FLJ90634 antibody
    • OTTHUMP00000022705 antibody
    • RP11-48C7.3 antibody
    • RRT2 antibody
    • WD repeat domain 85 antibody
    • WD repeat-containing protein 85 antibody
    • wdr85 antibody
    • WDR85_HUMAN antibody
    see all

Images

  • All lanes : Anti-WDR85 antibody (ab150815) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique.

    Predicted band size: 51 kDa

  • Immunohistochemical analysis of paraffin embedded Human breast tissue labeling WDR85 with ab150815 antibody at a dilution of 1/20.

References

ab150815 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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