SpecificityThis antibody recognizes ~72 kDa proteins, corresponding to the expected molecular weight of Wee1 on SDS-PAGE. The antibody sometimes detects ~30 kDa and ~45 kDa unknown bands in tissue extracts.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 18056802
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 72 kDa.
FunctionActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylated during M and G1 phases. Also autophosphorylated. Ubiquitinated and degraded at the onset of G2/M phase.