Overview

  • Product nameAnti-Wee1 antibody - Carboxyterminal end
    See all Wee1 primary antibodies
  • Description
    Rabbit polyclonal to Wee1 - Carboxyterminal end
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the sequence of human WEE1 within aa 600-700.

  • Positive control
    • HL-60 cell lysate (Western blot)
  • General notes


    This antibody recognises the C terminal of Wee 1.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab37597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated during M and G1 phases. Also autophosphorylated.
    Ubiquitinated and degraded at the onset of G2/M phase.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I18166 antibody
    • EC 2.7.10.2 antibody
    • FLJ16446 antibody
    • MGC105683 antibody
    • OTTHUMP00000231338 antibody
    • OTTHUMP00000231339 antibody
    • Wee 1 antibody
    • WEE 1 homolog 1 (S. pombe) antibody
    • WEE1 antibody
    • WEE1 homolog (S. pombe) antibody
    • Wee1 homolog antibody
    • WEE1 homolog S. pombe antibody
    • Wee1 like protein kinase antibody
    • Wee1 tyrosine kinase antibody
    • Wee1+ homolog antibody
    • Wee1+ S. pombe homolog antibody
    • WEE1, S. pombe, homolog of antibody
    • WEE1, somatic antibody
    • Wee1-like protein kinase antibody
    • WEE1_HUMAN antibody
    • WEE1A antibody
    • Wee1A kinase antibody
    • WEE1hu antibody
    see all

Anti-Wee1 antibody - Carboxyterminal end images

  • Anti-Wee1 antibody - Carboxyterminal end (ab37597) + HL-60 cell lysate

    Secondary
    HRP-anti-Rabbit
    Developed using the ECL technique

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 110 kDa,40 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of Wee1 expression in formalin-fixed, paraffin-embedded human breast carcinoma tissue sample using 1/50 ab37597.

References for Anti-Wee1 antibody - Carboxyterminal end (ab37597)

This product has been referenced in:
  • Butz H  et al. Down-Regulation of Wee1 Kinase by a Specific Subset of microRNA in Human Sporadic Pituitary Adenomas. J Clin Endocrinol Metab : (2010). Read more (PubMed: 20668041) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Non-functioning pituitary adenoma)
Loading amount 10 µg
Specification Non-functioning pituitary adenoma
Gel Running Conditions Reduced Denaturing (NuPAGE® 10% Bis-Tris Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"