Wee1 overexpression 293T lysate (whole cell) (ab94212)

Overview

  • Product nameWee1 overexpression 293T lysate (whole cell)
  • Description
    Wee1 overexpression lysate
  • General notesab94212 is a 293T cell transfected lysate in which Human Wee1 has been transiently over-expressed using a pCMV-Wee1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. Contains 1 protein kinase domain. PTM: Phosphorylated during M and G1 phases. Also autophosphorylated. Ubiquitinated and degraded at the onset of G2/M phase.

Applications

Our Abpromise guarantee covers the use of ab94212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Wee1 overexpression 293T lysate (whole cell) images

  • ab94212 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-Wee1 antibody (ab58059) at 1/500 dilution

    Lane 1 : Wee1 overexpression 293T lysate (whole cell) (ab94212)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Wee1 overexpression 293T lysate (whole cell) (ab94212)

ab94212 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"