• Product nameAnti-Wee1 (phospho S123) antibody
    See all Wee1 primary antibodies
  • Description
    Rabbit polyclonal to Wee1 (phospho S123)
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S123 of human Wee1.

  • Positive control
    • Human hepatocarcinoma tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab60034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.


  • FunctionActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated during M and G1 phases. Also autophosphorylated.
    Ubiquitinated and degraded at the onset of G2/M phase.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I18166 antibody
    • EC antibody
    • FLJ16446 antibody
    • MGC105683 antibody
    • OTTHUMP00000231338 antibody
    • OTTHUMP00000231339 antibody
    • Wee 1 antibody
    • WEE 1 homolog 1 (S. pombe) antibody
    • WEE1 antibody
    • WEE1 homolog (S. pombe) antibody
    • Wee1 homolog antibody
    • WEE1 homolog S. pombe antibody
    • Wee1 like protein kinase antibody
    • Wee1 tyrosine kinase antibody
    • Wee1+ homolog antibody
    • Wee1+ S. pombe homolog antibody
    • WEE1, S. pombe, homolog of antibody
    • WEE1, somatic antibody
    • Wee1-like protein kinase antibody
    • WEE1_HUMAN antibody
    • WEE1A antibody
    • Wee1A kinase antibody
    • WEE1hu antibody
    see all

Anti-Wee1 (phospho S123) antibody images

  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab60034 at a dilution of 1/50. A peroxidase-conjugated secondary antibody was used, followed by AEC staining.

References for Anti-Wee1 (phospho S123) antibody (ab60034)

This product has been referenced in:
  • Butz H  et al. Down-Regulation of Wee1 Kinase by a Specific Subset of microRNA in Human Sporadic Pituitary Adenomas. J Clin Endocrinol Metab : (2010). Read more (PubMed: 20668041) »

See 1 Publication for this product

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