Anti-Wee1 (phospho S53) antibody (ab111820)

Overview

  • Product name
    Anti-Wee1 (phospho S53) antibody
    See all Wee1 primary antibodies
  • Description
    Rabbit polyclonal to Wee1 (phospho S53)
  • Specificity
    ab111820 detects endogenous levels of Wee1 only when phosphorylated at serine 53.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from Human Wee1 around the phosphorylation site of Ser53 (E-D-SP-A-F).

  • Positive control
    • Human breast carcinoma tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111820 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated during M and G1 phases. Also autophosphorylated.
    Ubiquitinated and degraded at the onset of G2/M phase.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I18166 antibody
    • EC 2.7.10.2 antibody
    • FLJ16446 antibody
    • MGC105683 antibody
    • OTTHUMP00000231338 antibody
    • OTTHUMP00000231339 antibody
    • Wee 1 antibody
    • WEE 1 homolog 1 (S. pombe) antibody
    • WEE1 antibody
    • WEE1 homolog (S. pombe) antibody
    • Wee1 homolog antibody
    • WEE1 homolog S. pombe antibody
    • Wee1 like protein kinase antibody
    • Wee1 tyrosine kinase antibody
    • Wee1+ homolog antibody
    • Wee1+ S. pombe homolog antibody
    • WEE1, S. pombe, homolog of antibody
    • WEE1, somatic antibody
    • Wee1-like protein kinase antibody
    • WEE1_HUMAN antibody
    • WEE1A antibody
    • Wee1A kinase antibody
    • WEE1hu antibody
    see all

Images

  • ab111820 at 1/50 dilution staining Wee1 (phospho S53) in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with synthesized phosphopeptide.

References

ab111820 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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