Anti-Werner's syndrome helicase WRN antibody (ab17988)

Overview

  • Product nameAnti-Werner's syndrome helicase WRN antibody
    See all Werner's syndrome helicase WRN primary antibodies
  • Description
    Rabbit polyclonal to Werner's syndrome helicase WRN
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    The epitope recognised to a region between residues 1400 and the C-terminus (residue 1432) of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191.

  • Positive control
    • western blot on 293Tcells, whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at a concentration of 2 - 5 µg/ml.
    WB: 1/5,000 - 1/25,000. Detects a band of approximately 200 kDa (predicted molecular weight: 159 kDa).

    ab17988 does not seem to be suitable for ICC/IF - please refer to abreview for further details.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMultifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
    • Involvement in diseaseDefects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
      Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
    • Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
      Contains 1 3'-5' exonuclease domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HRDC domain.
    • Post-translational
      modifications
      Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus > nucleolus. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686C2056 antibody
      • DNA helicase antibody
      • DNA helicase, RecQ like type 3 antibody
      • Exonuclease WRN antibody
      • HGNC 12791 antibody
      • OTTHUMP00000225301 antibody
      • RecQ protein-like 2 antibody
      • RecQ-like type 3 antibody
      • RecQ3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • Werner syndrome ATP-dependent helicase antibody
      • Werner syndrome helicase antibody
      • Werner syndrome protein antibody
      • Werner syndrome, RecQ helicase like antibody
      • WRN antibody
      • WRN_HUMAN antibody
      see all

    Anti-Werner's syndrome helicase WRN antibody images

    • All lanes : Anti-Werner's syndrome helicase WRN antibody (ab17988) at 1/1000 dilution

      Lane 1 : Human Lymphocyte whole cell lysate
      Lane 2 : Human Lymphocyte whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      Anti Rabbit IgG conjugated to HRP at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 159 kDa


      Exposure time : 10 minutes

      This image is courtesy of an anonymous abreview.

      See Abreview

    • Detection of Human WRN by western blot and immunoprecipitation.

      Lane 1: 100 µg of whole cell lysate from 293T cells.
      Lane 2: 293T cell lysate immunoprecipated using IgG.
      Lane 3: 293T cell lysate immunoprecipitated using 5 µg of ab17987 + WRN blocking peptide.
      Lane 4: 293T cell lysate immunoprecipitated using 5 µg of ab17987.
      Lane 5: 293T cell lysate immunoprecipitated using 5 µg of ab17988 +WRN blocking peptide.
      Lane 6:  293T cell lysate immunoprecipitated using 5 µg of ab17988.

      Lanes 2-6 One 10 cm plate of 293T cells used for immunoprecipitation. 

      Anti-WRN antibody (ab17988) used at 1/10,000 for western blot.
      Detection:  Chemiluminescence with an exposure time of ~10 seconds.

    References for Anti-Werner's syndrome helicase WRN antibody (ab17988)

    This product has been referenced in:
    • Sallmyr A  et al. Up-regulation of WRN and DNA ligase III{alpha} in chronic myeloid leukemia: consequences for the repair of DNA double strand breaks. Blood : (2008). Read more (PubMed: 18524993) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (4-12%)
    Sample Human Cell lysate - whole cell (lymphocytes)
    Specification lymphocytes
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 05 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (RPE-1)
    Specification RPE-1
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Aug 16 2007

    Thank you for getting back in touch with me with your order details. I have arranged for a credit note to be raised by our accounts department against order numbers 126743 and 114423 for two vials of ab17988. They will be in touch with your purchas...

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    Thank you for your enquiry. In Charlotte's absence I am dealing with her enquiries. I am sorry to hear that you have been having difficulties with these antibodies. I have been reading through your technical questionaires and I appreciate the de...

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    Thank you for your enquiry. You ask an interesting question. I have been in discussion with the lab and they have referred me to the fact that the resolution of proteins by SDS-PAGE does not define molecular weight. It defines the relative mobilit...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"