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The epitope recognised to a region between residues 1400 and the C-terminus (residue 1432) of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191.
Our Abpromise guarantee covers the use of ab17988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This image is courtesy of an anonymous abreview.
Detection of Human WRN by western blot and immunoprecipitation.
Lane 1: 100
Lane 2: 293T cell lysate immunoprecipated using IgG.
Lane 3: 293T cell lysate immunoprecipitated using 5
Lane 4: 293T cell lysate immunoprecipitated using 5
Lane 5: 293T cell lysate immunoprecipitated using 5
Lane 6: 293T cell lysate immunoprecipitated using 5
Lanes 2-6 One 10 cm plate of 293T cells used for immunoprecipitation.
Anti-WRN antibody (ab17988) used at 1/10,000 for western blot.
Detection: Chemiluminescence with an exposure time of ~10 seconds.