Anti-Werner's syndrome helicase WRN (phospho S1141) antibody (ab74158)


  • Product nameAnti-Werner's syndrome helicase WRN (phospho S1141) antibody
    See all Werner's syndrome helicase WRN primary antibodies
  • Description
    Rabbit polyclonal to Werner's syndrome helicase WRN (phospho S1141)
  • Specificityab74158 detects endogenous levels of Werner's syndrome helicase WRN only when phosphorylated at serine 1141.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human Werner's syndrome helicase WRN around the phosphorylation site of serine 1141 (S-S-SP-Q-P).

  • Positive control
    • Extracts from K562 cells treated with etoposide (25uM, 24hours).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab74158 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab74158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 162 kDa.
ELISA 1/20000.


  • FunctionMultifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
  • Involvement in diseaseDefects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
    Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
    Contains 1 3'-5' exonuclease domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus > nucleolus. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686C2056 antibody
    • DNA helicase antibody
    • DNA helicase, RecQ like type 3 antibody
    • Exonuclease WRN antibody
    • HGNC 12791 antibody
    • OTTHUMP00000225301 antibody
    • RecQ protein-like 2 antibody
    • RecQ-like type 3 antibody
    • RecQ3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • Werner syndrome ATP-dependent helicase antibody
    • Werner syndrome helicase antibody
    • Werner syndrome protein antibody
    • Werner syndrome, RecQ helicase like antibody
    • WRN antibody
    • WRN_HUMAN antibody
    see all

Anti-Werner's syndrome helicase WRN (phospho S1141) antibody images

  • All lanes : Anti-Werner's syndrome helicase WRN (phospho S1141) antibody (ab74158) at 1/500 dilution

    Lane 1 : Extracts from K562 cells treated with etoposide (25uM, 24hours)
    Lane 2 : Extracts from K562 cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 162 kDa
    Observed band size : 162 kDa

References for Anti-Werner's syndrome helicase WRN (phospho S1141) antibody (ab74158)

ab74158 has not yet been referenced specifically in any publications.

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