The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 8 kDa.
Use at an assay dependent concentration. PubMed: 17634508
West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.
Envelope protein M antibody
Matrix protein antibody
West Nile virus matrix protein antibody
WNV matrix protein antibody
- West Nile Virus M glycoprotein antibody (ab22070)
IHC analysis of WNV-Envelope protein in bird liver using ab22070 at 1ug/ ml incubated for 30 minutes. A secondary conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. Image courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine
Western blot - West Nile Virus M glycoprotein antibody (ab22070)
Western blot analysis of West Nile Virus glycoprotein M in (A) untransfected and (B) transfected cells using ab22070 at 1 ug/ml.