Overview

  • Product name
  • Description
    Rabbit polyclonal to WHAMM
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: RALSSSSQAA THQNLGFRAP VKDDQPRPLV CESPAERPRD SLESFSCPGS MDEVLASLRH GRAPLRKVEV PAVRPPHASI NEHILAAIR, corresponding to internal sequence amino acids 660-748 of Human WHAMM.

  • Positive control
    • Human hippocampus tissue; A431 cells; Human liver, Human tonsil, RT 4 and U 251 MG lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500.

Target

  • Function
    Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex.
  • Tissue specificity
    Expressed in brain, lung, heart, colon and kidney (at protein level).
  • Sequence similarities
    Contains 2 WH2 domains.
  • Domain
    The N-terminal region associates with membranes, the coiled-coil region binds to microtubules and the WH2 domains promotes actin nucleation.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum-Golgi intermediate compartment. Cytoplasmic vesicle membrane. Golgi apparatus > cis-Golgi network. Localized to a perinuclear compartment near the microtubule-organizing center (MTOC). Also detected on tubulo-vesicular structures in the cell periphery that frequently localized along microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1971 antibody
    • membranes and microtubules antibody
    • WAS protein homolog associated with actin, golgi membranes and microtubules antibody
    • WAS protein homology region 2 domain containing 1 antibody
    • WAS protein homology region 2 domain-containing protein 1 antibody
    • WASP homolog-associated protein with actin antibody
    • WASP homolog-associated protein with actin, membranes and microtubules antibody
    • WH2 domain-containing protein 1 antibody
    • WHAMM antibody
    • WHAMM_HUMAN antibody
    • WHDC1 antibody
    see all

Images

  • ab122572, at 1/200 dilution, staining WHAMM in paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
  • ab122572, at 4 µg/ml, staining WHAMM in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
  • All lanes : Anti-WHAMM antibody (ab122572) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab122572 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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