Overview

  • Product nameAnti-WHAMM antibody
    See all WHAMM primary antibodies
  • Description
    Rabbit polyclonal to WHAMM
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: EELKVIDCVV GLQDDKNLEV KELRRQCQQL ESKRGRICAK RASLRSRKDQ CKENHRFRLQ QAEESIRYSR QHH, corresponding to amino acids 439-511 of Human WHAMM.

  • Positive control
    • Human lateral ventricle tissue; U 2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab122639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • FunctionActs as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex.
  • Tissue specificityExpressed in brain, lung, heart, colon and kidney (at protein level).
  • Sequence similaritiesContains 2 WH2 domains.
  • DomainThe N-terminal region associates with membranes, the coiled-coil region binds to microtubules and the WH2 domains promotes actin nucleation.
  • Cellular localizationCytoplasm. Endoplasmic reticulum-Golgi intermediate compartment. Cytoplasmic vesicle membrane. Golgi apparatus > cis-Golgi network. Localized to a perinuclear compartment near the microtubule-organizing center (MTOC). Also detected on tubulo-vesicular structures in the cell periphery that frequently localized along microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1971 antibody
    • membranes and microtubules antibody
    • WAS protein homolog associated with actin, golgi membranes and microtubules antibody
    • WAS protein homology region 2 domain containing 1 antibody
    • WAS protein homology region 2 domain-containing protein 1 antibody
    • WASP homolog-associated protein with actin antibody
    • WASP homolog-associated protein with actin, membranes and microtubules antibody
    • WH2 domain-containing protein 1 antibody
    • WHAMM antibody
    • WHAMM_HUMAN antibody
    • WHDC1 antibody
    see all

Anti-WHAMM antibody images

  • ab122639, at 1/5, staining WHAMM in paraffin embedded Human lateral ventricle tissue by Immunohistochemistry.
  • ab122639, at 4 µg/ml, staining WHAMM in in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).

References for Anti-WHAMM antibody (ab122639)

ab122639 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122639.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"