Anti-Wheat Germ Agglutinin antibody (FITC) (ab20528)

Overview

  • Product name
    Anti-Wheat Germ Agglutinin antibody (FITC)
    See all Wheat Germ Agglutinin primary antibodies
  • Description
    Rabbit polyclonal to Wheat Germ Agglutinin (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC/IF, Immunodiffusion, Immunoelectrophoresismore details
  • Species reactivity
    Reacts with: Triticum aestivum
  • Immunogen

    Wheat germ agglutinin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 40% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 20 - 100 µg/ml.
IHC-P Use a concentration of 20 - 100 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 100 µg/ml.
Immunodiffusion Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.

Target

  • Relevance
    WGA is a N acetylglucosamine specific lectin and is synthesized in developing and germinating embryos, roots and meristematic part of the leaves. Lectin interaction with certain carbohydrates are as specific as enzyme to substrate or antigen to antibody interactions and have been used to study protein glycosylation. WGA has been reported to have a role in dormancy and plant defence, but its characteristic biochemical properties and its predominance in growing and meristematic tissues, and its osmotic- and drought-stress inducibility suggest that this protein might be involved in a much wider range of functions.
  • Alternative names
    • Agglutinin isolectin 1 antibody
    • Agglutinin isolectin 2 antibody
    • Agglutinin isolectin 3 antibody
    • isolectin antibody
    • Lectin antibody
    • Triticum aestivum antibody
    • Triticum vulgaris agglutinin antibody
    • WGA antibody
    • Wheat germ agglutinin antibody
    see all

References for Anti-Wheat Germ Agglutinin antibody (FITC) (ab20528)

ab20528 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Kidney)
Permeabilization
No
Specification
Kidney
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

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Submitted Feb 01 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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