The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 72 kDa.
FunctionFunctions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the AAA ATPase family. Contains 1 UBZ-type zinc finger.
Post-translational modificationsSumoylated by SUMO1 and SUMO2/3.
Cellular localizationNucleus. Colocalizes with WRN in granular structures in the nucleus.