Overview

  • Product nameAnti-WHIP antibody
    See all WHIP primary antibodies
  • Description
    Rabbit polyclonal to WHIP
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 400 and 450 of Human WHIP (NP_064520.2).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionFunctions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the AAA ATPase family.
    Contains 1 UBZ-type zinc finger.
  • Post-translational
    modifications
    Sumoylated by SUMO1 and SUMO2/3.
  • Cellular localizationNucleus. Colocalizes with WRN in granular structures in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATPase WRNIP 1 antibody
    • ATPase WRNIP1 antibody
    • bA420G6.2 antibody
    • FLJ22526 antibody
    • Putative helicase RUVBL antibody
    • RP11 420G6.2 antibody
    • Werner helicasae interacting protein 1 antibody
    • Werner helicasae interacting protein antibody
    • Werner helicase interacting protein isoform 2 antibody
    • Werner helicase-interacting protein 1 antibody
    • WRIP1_HUMAN antibody
    • WRNIP 1 antibody
    • WRNIP antibody
    • Wrnip1 antibody
    see all

Anti-WHIP antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling WHIP with ab99316 at 1/1000 (1µg/ml). Detection: DAB.
  • Anti-WHIP antibody (ab99316) at 1/1000 dilution + Mouse T cell lysate at 80 µg

    Secondary
    IRDye® 680-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilution

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-WHIP antibody (ab99316) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 69 kDa


    Exposure time : 30 seconds
  • Detection of WHIP by Western Blot of Immunprecipitate.
    ab99316, at 1 µg/ml, staining WHIP in HeLa whole cell lysate immunoprecipitated using ab99316 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-WHIP antibody (ab99316)

ab99316 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 80 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (T cell)
Specification T cell
Blocking step LI-COR blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Aug 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"