- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-WHIP antibody
See all WHIP primary antibodies -
DescriptionRabbit polyclonal to WHIP
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between amino acids 400 and 450 of Human WHIP (NP_064520.2).
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Positive control
- HeLa whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab99316 was affinity purified using an epitope specific to WHIP immobilized on solid support
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab99316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 69 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionFunctions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
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Tissue specificityUbiquitously expressed.
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Sequence similaritiesBelongs to the AAA ATPase family.
Contains 1 UBZ-type zinc finger. -
Post-translational
modificationsSumoylated by SUMO1 and SUMO2/3. -
Cellular localizationNucleus. Colocalizes with WRN in granular structures in the nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 56897 Human
- Entrez Gene: 78903 Mouse
- Entrez Gene: 282835 Rat
- Omim: 608196 Human
- SwissProt: Q96S55 Human
- SwissProt: Q91XU0 Mouse
- SwissProt: Q8CG07 Rat
- Unigene: 236828 Human
see all -
Alternative names
- ATPase WRNIP 1 antibody
- ATPase WRNIP1 antibody
- bA420G6.2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHIP antibody (ab99316)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling WHIP with ab99316 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - Anti-WHIP antibody (ab99316)This image is courtesy of an anonymous AbreviewAnti-WHIP antibody (ab99316) at 1/1000 dilution + Mouse T cell lysate at 80 µg
Secondary
IRDye® 680-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa (why is the actual band size different from the predicted?)
Exposure time: 5 minutes
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Western blot - Anti-WHIP antibody (ab99316)All lanes : Anti-WHIP antibody (ab99316) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-WHIP antibody (ab99316)Detection of WHIP by Western Blot of Immunprecipitate.
ab99316, at 1 µg/ml, staining WHIP in HeLa whole cell lysate immunoprecipitated using ab99316 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
References
ab99316 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"