Customer reviews and FAQs

Overview

  • Product name
    Anti-WHIP antibody
    See all WHIP primary antibodies
  • Description
    Rabbit polyclonal to WHIP
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 400 and 450 of Human WHIP (NP_064520.2).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the AAA ATPase family.
    Contains 1 UBZ-type zinc finger.
  • Post-translational
    modifications
    Sumoylated by SUMO1 and SUMO2/3.
  • Cellular localization
    Nucleus. Colocalizes with WRN in granular structures in the nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ATPase WRNIP 1 antibody
    • ATPase WRNIP1 antibody
    • bA420G6.2 antibody
    • FLJ22526 antibody
    • Putative helicase RUVBL antibody
    • RP11 420G6.2 antibody
    • Werner helicasae interacting protein 1 antibody
    • Werner helicasae interacting protein antibody
    • Werner helicase interacting protein isoform 2 antibody
    • Werner helicase-interacting protein 1 antibody
    • WRIP1_HUMAN antibody
    • WRNIP 1 antibody
    • WRNIP antibody
    • Wrnip1 antibody
    see all

Anti-WHIP antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHIP antibody (ab99316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHIP antibody (ab99316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling WHIP with ab99316 at 1/1000 (1µg/ml). Detection: DAB.
  • Western blot - Anti-WHIP antibody (ab99316)
    Western blot - Anti-WHIP antibody (ab99316)This image is courtesy of an anonymous Abreview
    Anti-WHIP antibody (ab99316) at 1/1000 dilution + Mouse T cell lysate at 80 µg

    Secondary
    IRDye® 680-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilution

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Western blot - WHIP antibody (ab99316)
    Western blot - WHIP antibody (ab99316)
    All lanes : Anti-WHIP antibody (ab99316) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 69 kDa


    Exposure time : 30 seconds
  • Immunoprecipitation - WHIP antibody (ab99316)
    Immunoprecipitation - WHIP antibody (ab99316)
    Detection of WHIP by Western Blot of Immunprecipitate.
    ab99316, at 1 µg/ml, staining WHIP in HeLa whole cell lysate immunoprecipitated using ab99316 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-WHIP antibody (ab99316)

ab99316 has not yet been referenced specifically in any publications.

Publishing research using ab99316? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-WHIP antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (T cell)
Specification
T cell
Blocking step
LI-COR blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Aug 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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