The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/400 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).
Use at 1/400 for for tissue extracts/cell lysates, and at 1/1000 for recombinant protein.
RelevanceWhite spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. The VP28 was one of the envelope proteins of WSSV. Almost all shrimps infected with WSSV in ponds die within 3 to 4 days after the first dead shrimp was observed with gross lesions ranging from abnormal red body discoloration to white spots in the cuticle.
Western blot - White spot syndrome virus VP28 antibody (ab26935)
All lanes : Anti-White spot syndrome virus VP28 antibody (ab26935) at 1/1000 dilution
Lane 1 : Lane (A M1) Control: E.Coli total cell lysate before IPTG induction, Pre bleed Lane 2 : Lane (A M2) Recombinant VP28 protein expression after IPTG induction, Pre Bleed Lane 3 : Lane (B M1) Control: E.Coli total cell lysate before IPTG induction, Post immunization. Lane 4 : Lane (B M2) Recombinant VP28 protein expression after IPTG induction, Post immunization.