• Product name
  • Description
    Rabbit polyclonal to WHSC1/NSD2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Dog
  • Immunogen

    Synthetic peptide, corresponding to amino acids 219-268 of Human WHSC1

  • Positive control
    • HepG2 cell lysate.



Our Abpromise guarantee covers the use of ab28470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.


  • Function
    Probable histone methyltransferase (By similarity). May act as a transcription regulator that binds DNA and suppresses IL5 transcription.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Note=A chromosomal aberration involving WHSC1 is a cause of multiple myeloma tumors. Translocation t(4;14)(p16.3;q32.3) with IgH.
    Note=WHSC1 is located in the Wolf-Hirschhorn syndrome (WHS) critical region. WHS results from by sub-telomeric deletions in the short arm of chromosome 4. WHSC1 is deleted in every case, however deletion of linked genes contributes to both the severity of the core characteristics and the presence of the additional syndromic problems.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 1 HMG box DNA-binding domain.
    Contains 4 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 2 PWWP domains.
    Contains 1 SET domain.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm and Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ23286 antibody
    • IL5 promoter REII region binding protein antibody
    • KIAA1090 antibody
    • MGC176638 antibody
    • MMSET antibody
    • MMSET type II antibody
    • Multiple myeloma SET domain containing protein type III antibody
    • Multiple myeloma SET domain protein antibody
    • Multiple myeloma SET domain-containing protein antibody
    • NSD 2 antibody
    • NSD2 antibody
    • NSD2_HUMAN antibody
    • Nuclear receptor binding SET domain protein 2 antibody
    • Nuclear SET domain-containing protein 2 antibody
    • Probable histone-lysine N-methyltransferase NSD2 antibody
    • Protein trithorax-5 antibody
    • REIIBP antibody
    • Trithorax/ash1 related protein 5 antibody
    • TRX5 antibody
    • TRX5 protein antibody
    • WHS antibody
    • Whsc1 antibody
    • Wolf Hirschhorn syndrome candidate 1 antibody
    • Wolf Hirschhorn syndrome candidate 1 protein antibody
    • Wolf-Hirschhorn syndrome candidate 1 protein antibody
    see all

Anti-WHSC1/NSD2 antibody images

  • Anti-WHSC1/NSD2 antibody (ab28470) at 0.5 µg/ml + 5% skim milk / PBS buffer.
    Developed using the ECL technique

    Predicted band size : 72 kDa

References for Anti-WHSC1/NSD2 antibody (ab28470)

This product has been referenced in:
  • Hajdu I  et al. Wolf-Hirschhorn syndrome candidate 1 is involved in the cellular response to DNA damage. Proc Natl Acad Sci U S A 108:13130-4 (2011). WB ; Human . Read more (PubMed: 21788515) »

See 1 Publication for this product

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I am very pleased to hear you would like to accept our offer and test ab28470 in mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the &...

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To our knowledge, ab28470 has not been tested in mouse. We predict it to work in mouse due to high sequence homology, but we do not guarantee it since we haven't tested this. Therefore, I can o...

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Western blot
Human Cell lysate - whole cell (Hela & HCT116 cells)
Loading amount
50 µg
Hela & HCT116 cells
Gel Running Conditions
Reduced Denaturing (10% tris glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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Verified customer

Submitted May 08 2012


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