Overview

  • Product nameAnti-WHSC2 antibody
    See all WHSC2 primary antibodies
  • Description
    Mouse polyclonal to WHSC2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human WHSC2 protein (NP_005654.2).

  • Positive control
    • WB: Jurkat cell lysate; WHSC2 transfected 293T cell lysate ICC/IF: HeLa cell

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionEssential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.
  • Tissue specificityUbiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.
  • Sequence similaritiesBelongs to the NELF-A family.
    Contains 1 HDAg-like domain.
  • DomainThe HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10442 antibody
    • FLJ25112 antibody
    • Negative elongation factor A antibody
    • Negative elongation factor polypeptide A antibody
    • NELF A antibody
    • NELF-A antibody
    • NELFA antibody
    • NELFA_HUMAN antibody
    • P/OKcl.15 antibody
    • P/OKcl15 antibody
    • WHSC 2 antibody
    • Whsc2 antibody
    • Wolf Hirschhorn syndrome candidate 2 antibody
    • Wolf Hirschhorn syndrome candidate 2 protein antibody
    • Wolf-Hirschhorn syndrome candidate 2 protein antibody
    see all

Anti-WHSC2 antibody images

  • Anti-WHSC2 antibody (ab88870) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • All lanes : Anti-WHSC2 antibody (ab88870) at 1 µg/ml

    Lane 1 : WHSC2 transfected 293T cell lysate
    Lane 2 : Non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 58 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab88870, at 10 µg/ml, staining WHSC2 in HeLa cell by Immunofluorescence.

References for Anti-WHSC2 antibody (ab88870)

ab88870 has not yet been referenced specifically in any publications.

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