The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
Use a concentration of 10 µg/ml.
FunctionEssential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.
Tissue specificityUbiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.
Sequence similaritiesBelongs to the NELF-A family. Contains 1 HDAg-like domain.
DomainThe HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex.