WI-38 nuclear extract lysate (ab14911)

Overview

  • Product nameWI-38 nuclear extract lysate
    See all WI 38 lysates
  • General notesTo ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC. The protein content has been determined by a Bradford-based assay.

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.50
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT
    Note: With Protease inhibitor
  • Concentration information loading...
  • Research areas

    WI-38 nuclear extract lysate images

    • Each lot has been tested for AP-1 activation by using TransAM™ AP-1 Kits. The signal intensity for AP-1 activation in each lot is compared to the signal intensity obtained with extracts from unstimulated WI-38 VA13 cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4 when using TransAM AP-1 c-Fos. This ratio may vary depending on the basal level of AP-1 activation in a given cell type.

    References for WI-38 nuclear extract lysate (ab14911)

    ab14911 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"