Each lot has been tested for AP-1 activation by using TransAM™ AP-1 Kits. The signal intensity for AP-1 activation in each lot is compared to the signal intensity obtained with extracts from unstimulated WI-38 VA13 cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4 when using TransAM AP-1 c-Fos. This ratio may vary depending on the basal level of AP-1 activation in a given cell type.
References for WI-38 nuclear extract lysate (ab14911)
has not yet been referenced specifically in any publications.
Publishing research using ab14911? Please let us know so that we can cite the reference in this datasheet.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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