Each lot has been tested for AP-1 activation by using TransAM™ AP-1 Kits. The signal intensity for AP-1 activation in each lot is compared to the signal intensity obtained with extracts from unstimulated WI-38 VA13 cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4 when using TransAM AP-1 c-Fos. This ratio may vary depending on the basal level of AP-1 activation in a given cell type.
References for WI-38 nuclear extract lysate (ab14911)
has not yet been referenced specifically in any publications.
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