WI-38 nuclear extract lysate (TPA + CI stimulated) (ab14913)

Overview

  • Product nameWI-38 nuclear extract lysate (TPA + CI stimulated)
    See all WI 38 lysates
  • Description
    WI-38 nuclear extract lysate
  • General notesTo ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC. The protein content has been determined by a Bradford-based assay. The WI-38 nuclear extracts have been collected after a 2-hour incubation with or without TPA (100 ng/ml) and ionomycin (calcium ionophore) (2 µg/ml).

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.50
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT
    Note: With Protease inhibitors
  • Concentration information loading...
  • Research areas

    References for WI-38 nuclear extract lysate (TPA + CI stimulated) (ab14913)

    ab14913 has not yet been referenced specifically in any publications.

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