Anti-wide spectrum Cytokeratin antibody (ab9377)

Overview

  • Product nameAnti-wide spectrum Cytokeratin antibody
    See all wide spectrum Cytokeratin primary antibodies
  • Description
    Rabbit polyclonal to wide spectrum Cytokeratin
  • SpecificityThis antibody reacts with cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68 kDa.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, Pig, Cynomolgus Monkey, Marmoset (common)
  • Immunogen

    Cytokeratin isolated from bovine muzzle epidermis.

Properties

Applications

Our Abpromise guarantee covers the use of ab9377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Used at a dilution of 1/1000 overnight incubation (see Abreview for further information).
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. This antibody may be diluted to a titer of 1:75-1:200 in an ABC method.
IHC-Fr Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.

Target

  • Alternative names
    • pan cytokeratin antibody

Anti-wide spectrum Cytokeratin antibody images

  • ab9377 staining wide spectrum Cytokeratin in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry.
  • ab14404 staining wide spectrum Cytokeratin in the Human urether cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200) for 16 hours. A Cy3®-conjugated Goat anti-rabbit polyclonal (1/800) was used as the secondary antibody.

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  • ab17976 staining wide spectrum Cytokeratin in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with BlockAid for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/500 in 1% BSA + PBS) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.

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  • ab9377 staining wide spectrum Cytokeratin in rat liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.

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  • Ab9377 positively staining paraformaldehyde fixed human retinal pigment epithelium derived from a fetal donor and established in primary culture(1/75). Ab9377 was used in conjunction with goat anti rabbit Alexa Ò488 (1/100).

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  • ab9377 staining wide spectrum Cytokeratin in common marmoset lung tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.

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  • Ab9377 positively staining Cytokeratin (green) in paraffin fixed mouse oesophagus at 1/250.

     

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  • ab9377 staining mouse mesonephric epithelium cells by ICC/IF. Cells were methanol fixed and blocked in 1% BSA, 2% FBS in PBS for 20 minutes at 23°C prior to incubating with the primary antibody, diluted 1/75, for 18 hours at 4°C.  An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody was used as the secondary.

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References for Anti-wide spectrum Cytokeratin antibody (ab9377)

This product has been referenced in:
  • Crowley C  et al. Surface modification of a POSS-nanocomposite material to enhance cellular integration of a synthetic bioscaffold. Biomaterials 83:283-93 (2016). IHC-P ; Human . Read more (PubMed: 26790147) »
  • Judd LM  et al. Elevated IL-33 expression is associated with pediatric eosinophilic esophagitis, and exogenous IL-33 promotes eosinophilic esophagitis development in mice. Am J Physiol Gastrointest Liver Physiol 310:G13-25 (2016). Mouse . Read more (PubMed: 26514775) »

See all 36 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Liver
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Bronchus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Bronchus
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Liver
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin and colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Skin and colon
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 24 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat Placenta)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Rat Placenta
Blocking step Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 26 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Hamster Tissue sections (Uterus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization No
Specification Uterus
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 24 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BlockAid as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
Sample Human Tissue sections (Skin)
Specification Skin
Permeabilization Yes - 0.2% Triton X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 06 2015

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