Anti-WIF1 antibody (ab33281)

Overview

  • Product nameAnti-WIF1 antibody
    See all WIF1 primary antibodies
  • Description
    Rabbit polyclonal to WIF1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human WIF1.

    (Peptide available as ab33600.)

  • Positive control
    • ab33281 gave a positive signal in the Human WIF-1 recombinant.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab33281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/250. Detects a band of approximately 46 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human WIF1 peptide (ab33600).

Target

Anti-WIF1 antibody images

  • ab33281 staining WIF1 in Mouse MEF cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 5% Goat serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in 0.5% Triton X-100) for 2 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Anti-WIF1 antibody (ab33281) at 1/250 dilution + WIF-1 Recombinant Protein at 0.0025 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    The band we observe at 46 kDa is believed to be the glycosylated form of the recombinant protein.

References for Anti-WIF1 antibody (ab33281)

ab33281 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Hair follicle)
Permeabilization No
Specification Hair follicle
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Ahmar Aziz

Verified customer

Submitted May 19 2015

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (hek293, overexpression of hWIF1)
Specification hek293, overexpression of hWIF1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted May 20 2013

Thank you very much for your interest in ab33281. To our knowledge, ab33281 has not been tested in immunofluorescence. Therefore, I can offer a discount off a future purchase if you buy ab33281 now, test it in immunofluorescence and submit feedback to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"